Extraction of Benzoylecgonine from Urine - CORE - benzoylecgonine detection time in urine

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• (1) benzoylecgonine positive but neg cocaine
• (2) benzoylecgonine cocaine metabolite urine
• (3) cocaine metabolite detection times
• (4) benzoylecgonine urine test

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John Wesley Powell Student Research 2002, 13th Annual JWP Conference
Conference
Apr 21st, 1:15 PM - 2:15 PM
Extraction of Benzoylecgonine from Urine
Jeffrey T. Woodruff, '02
Illinois Wesleyan University
David Bailey, Faculty Advisor
Illinois Wesleyan University
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Jeffrey T. Woodruff, '02 and David Bailey, Faculty Advisor, "Extraction of Benzoylecgonine from Urine" (April 21, 2002).
John Wesley Powell Student Research Conference. Paper 1.
http://digitalcommons.iwu.edu/jwprc/2002/posters3/1
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u
Poster Presentation P52
EXTRACTION OF BENZOYLECGONINE FROM URINE
Departm
Jeffrey T. Woodruff and David B ailey*
ent of Chemistry, lllinois Wesleyan University
A method for extracting Benzoylecgonine (BE) from urine is being developed which
p
li
r
C
tilizes UV-vis spectroscopy as a means to determine cocaine abuse. BE is the most
e
f
is
b
evalent metabolite formed from the degradation of cocaine in the body. It has a half?
e six times as long as that of cocaine, and can thus be detected in the body for longer
h
Co
B
t
riods of time. The most common method of BE detection used currently is Gas
a
ei
fr
p
g
romatographylMass Spectrometry. However, due to the derivatization involved, this
ns
costly method. Other methods have not been used because BE being is a zwitterion.
ng
equently, it is very hydrophilic and is difficult to extract from urine. A method is
E int
developed in which hydrochloric acid is added to a solution of BE in urine to force
o its cationic state. It can then be ion-paired with selected anions and extracted
om urine using methylene chloride. A UV-vis spectrum is then obtained. This provides
he experimenter with a positive or negative reading for cocaine use. If the reading is
sp
ositive, further testing can be run utilizing GCIMS. However, by utilizing a UV-vis
re
ectrophotometer to obtain a positive or negative reading, the cost of drug testing is
atly reduced.


How long does benzoylecgonine stay in your urine? Well benzoylecgonine which is the metabolite of cocaine can be detected in your urine for upto 5 days if you are NOT a regular user. This would mean that you have used cocaine occasionally or just the once and in such a scenario you would have the metabolite in your urine for a maximum of 5 days.

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