Home / convert mg ml to molarity / How to calculate molarity - Virginia Commonwealth …

How to calculate molarity - Virginia Commonwealth … - convert mg ml to molarity

How to calculate molarity - Virginia Commonwealth …-convert mg ml to molarity

How to make solutions and calculate molarity
A. Why make solutions and calculate molarity?
If you have taken labs courses, you probably had solutions handed to you. In real life, you make your
solutions yourself. To function in a lab, you therefore need to be able to figure out what to weigh out on
your own.
B. Typical example #1: Fixed volume, adjustable weight
The usual situation is that you're following a recipe that specifies, for example, a 0.5 molar solution of
NaOH. You know that "0.5 M" literally means 0.5 moles per liter. Unfortunately:
You can't weigh out moles.
You don't need a liter. 200 ml is more than enough.
Let's pause and assess what you have before you. You know that you will make 200 ml of a NaOH solution
that is 0.5 M. You know that to do this, you will weigh out so many grams of NaOH and add water to
dissolve it, ending up with 200 ml. Do you have enough information to figure out how many grams to
Units are our friends. Let's ask them for help:
You know the end molarity (0.5 M NaOH), which has units of moles / volume
You know the end volume (200 ml)
You should be able to calculate the number of moles of NaOH in the solution:
(moles / volume) * (end volume) = moles
So getting the number of moles is straightforward, but what you want is the weight.
You could go from moles to weight if you had some quantity with units of weight/mole:
(moles) * (weight/mole) = weight
At this point, you should recognize that weight/mole is the definition of molecular weight. You can find the
molecular weight of a compound most readily by taking a look at the manufacturer's bottle from which you
will be taking the compound. You will almost always see "MW=..." or "FW=..." ("FW" means "free
weight" and corresponds to the molecular weight of the compound plus any molecules of water that may
precipitate with it). If you don't find it in the bottle, there's many other places to look (a manufacturers
catalog, a chemical index,...). Suppose you get the molecular weight of NaOH from one of these sources:
it's 40.0 g/mole. Now, plugging in:
(0.5 moles/liter NaOH) * (200 ml end volume) * (40.0 g/mole) = amount to weigh out
Problem: the units you get from this equation (after canceling) is: (ml/liter) * g. In order to get grams, you'll
have to convert one of the volume units. I'll do it this way:
(0.5 moles/liter NaOH) * (0.2 liter end volume) * (40.0 g/mole) = amount to weigh out
Now the units cancel, except for grams, giving 4.00 g.
SQ1. How much MgSO4 ? 7 H2O do you need to weigh out to get 150 ml of a 10 mM solution? The free
weight is 246.5 g/mol.
B. Typical example #2: Adjustable volume, fixed weight
When you weight out tiny amounts of potent and often very expensive chemicals, it is often impossible to
get exactly the weight you were hoping for. For example, suppose you want to make a 2 mg/ml stock
solution of an enzyme and you only need about 1 ml. That's easy: (2 mg/ml)*(1ml)... you need 2 mg.
Unfortunately, it is not easy to weigh out exactly 2 mg, and you weigh out 2.4 mg instead. Don't bother
trying to take away the extra 0.4 mg... you'll be there for hours. Instead, change the volume so that the
concentration ends up as you wanted, using all of the 2.4 mg.
SQ2. What volume water should you add to 2.4 mg of the enzyme to get a concentration of 2 mg/ml?
C. Percent solutions
Sometimes you need to make up a solution not by concentration or molarity but by percent, e.g. 20%
sucrose or 15% glycerol solutions. Recipes are supposed to add the definition of percent, e.g. 20% sucrose
w/v (weight per volume) or 15% glycerol v/v (volume per volume). The first case means that you should end
up with 20 g sucrose in 100 ml of end solution. The second case means that you should end up with 15 ml
glycerol in 100 ml of end solution. Of course you wouldn't dream of adding 100 ml of water to the sucrose
or glycerol, since that would give you an end solution greater than 100 ml (since the solute has volume too).

What is the formula for calculating molarity? Molarity Formula. The equation to calculate the molarity is the ratio of the moles of solute whose molarity is to be calculated and the volume of solvent used to dissolve the given solute. (M=frac{n}{V}) Here, M is the molality of the solution that is to be calculated. n is the number of moles of the solute